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Title 

Novel induction of alpha-lactalbumin-mediated lacdiNAc-R expression in vivo

Authors 

Su Il DoKi-Young LeeHee Nam Kim

Publisher 

Portland Press

Issue Date 

2000

Citation 

Biochemical Journal, vol. 348, no. 1, pp. 229-234

Keywords 

nucleotide sugar switchingLec8 cellsβ1,4-galactosyltransferaseβ1,4-N-acetylgalactosaminyltransferase

Abstract 

alpha-Lactalbumin (alpha-LA) is a regulatory protein by which the mammalian beta1,4-galactosyltransferase (beta1,4-galT) is induced to utilize glucose as an acceptor instead of N-acetylglucosamine (GlcNAc) during lactose synthesis in mammary gland. alpha-LA can also modulate beta1,4-galT to utilize UDP-N-acetylgalactosamine (UDP-GalNAc) as a donor towards GlcNAc acceptor substrate with high efficiency in vitro [Do, Do and Cummings (1995) J. Biol. Chem. 270, 18447-18451]. In the present study we transfected cDNA encoding bovine alpha-LA into Lec8 cells and examined whether nucleotide sugar switching of UDP-galactose (UDP-Gal) into UDP-GalNAc occurred in vivo and whether the neo-glycosylation of GalNAcbeta1,4GlcNAc-R structure was synthesized in alpha-LA-stable transfectants. Our studies demonstrate that the stable expression of alpha-LA in Lec8 cells induces the formation of GalNAcbeta1,4GlcNAc-R in vivo through the nucleotide sugar switching of beta1,4-galT.

ISSN 

0264-6021

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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