상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Involvement of SH2-SH2-SH3 domain of phospholipase cγ1 in NF-κB signaling

Authors 

Bo Yeon KimDae Ook KangWon Keun OhJin Hee KimYong Kyung ChoeJong Soo JangPann Gill SuhSung Ho RyuTae Ick MheenJong Seog Ahn

Publisher 

Elsevier

Issue Date 

2000

Citation 

FEBS Letters, vol. 472, no. 1, pp. 45-49

Keywords 

nuclear factor κBphospholipase Cγ1protein kinase CPhosphatidylinositol 3-kinasemitogen activated protein kinasephosphatidylinositol 3-kinaseSH2-SH3

Abstract 

To directly define the role of phospholipase Cγ1 (PLCγ1) in NF-κB activation, NF-κB promoted luciferase reporter gene plasmid (pNF-κB-Luc) was transfected into rat-3Y1 fibroblasts that overexpress whole PLCγ1 (PLCγ1-3Y1), src homology domains SH2-SH2-SH3 of PLCγ1 (SH223-3Y1) and v-src (Src-3Y1). Transient transfection with pNF-κB-Luc remarkably increased the luciferase activity in all three transformants compared with normal rat-3Y1 cells. Pretreatment with inhibitors of protein tyrosine kinase reduced this increase in luciferase activity, but U73122 (a PLC inhibitor) did not. While PD98059, an inhibitor of mitogen activated protein kinase (MAPK), significantly reduced the luciferase activity, there was no effect by wortmannin and Ro-31-8220, inhibitors of phosphatidylinositol 3-kinase (PI3K) and protein kinase C (PKC), respectively. This study shows a direct evidence that the SH2-SH2-SH3 region of PLCγ1 contributes to the NF-κB signaling and that MAPK, but not PI3K and PKC, is involved in SH2-SH2-SH3 mediated NF-κB activation in these cells.

ISSN 

0014-5793

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)