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Title 

Specific binding of recombinant Listeria monocytogenes p60 protein to Caco-2 cells

Authors 

Jung Hyun ParkYong Soon LeeYoon Kyu LimSoon Hwan KwonChil U LeeByoung Soo Yoon

Publisher 

Wiley-Blackwell

Issue Date 

2000

Citation 

FEMS Microbiology Letters, vol. 186, no. 1, pp. 35-40

Keywords 

maltose binding proteininvasion associated proteinp60recombinant proteinListeria monocytogenes

Abstract 

The Listeria monocytogenes p60 is a major extracellular protein, which is believed to be involved in the invasion of these bacteria into their host cells. So far the mechanism by which p60 participates in the internalization or penetration of L. monocytogenes is still veiled. To determine the possibility of a direct interaction of p60 with the host cell surface, the iap gene was recombinantly expressed in Escherichia coli and used for binding studies with the enterocyte-like Caco-2 cells. Fluorescence activated flow cytometry and confocal laser scanning microscopy revealed a cell membrane specific staining with p60, which implications in Listeria virulence are discussed.

ISSN 

0378-1097

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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