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Title 

Fine mapping of virus-neutralizing epitopes on hepatitis B virus PreS1

Authors 

Cheol Young MaengChun Jeih RyuPhilippe GriponChristiane Guguen-GuillouzoHyo Jeong Hong

Publisher 

Elsevier

Issue Date 

2000

Citation 

Virology, vol. 270, no. 1, pp. 9-16

Keywords 

hybrid proteinmonoclonal antibodymonoclonal antibody KR 127monoclonal antibody KR 259neutralizing antibodyantigen bindingepitope mappingHepatitis B virusvirus immunityvirus neutralization

Abstract 

We identified the epitopes on the preS1 which induce antibodies that neutralize both ad and ay subtypes of hepatitis B virus (HBV). Previously we generated murine monoclonal antibodies KR359 and KR127 that bind specifically to the preS1 of HBV. In this study we have performed fine mappings of the epitopes of the antibodies by examining their reactivity with GST fusion proteins, which contain a series of deletion mutants of the preS1. KR359 and KR127 specifically recognize aa 19-26 and 37-45 of the preS1, respectively. The antibodies neutralized both adr and ayw subtypes of the virus in an in vitro neutralization assay using in vitro infection of adult human hepatocyte primary culture by HBV. The epitopes showed little sequence divergence and the antibodies bound to the preS1 of all the HBV subtypes and variants tested.

ISSN 

0042-6822

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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