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Title 

Synthesis of recombinant human interleukin-2 via controlled feed of lactose-complex media in fed-batch cultures of Escherichia coli BL21(DE3)[pT7-G3IL2]

Authors 

Vibhor SaraswatJee Won LeeDae Young KimYoung Hoon Park

Publisher 

Springer Verlag (Germany)

Issue Date 

2000

Citation 

Biotechnology Letters, vol. 22, no. 4, pp. 261-265

Keywords 

Escherichia coliInterleukin-2lactosesymport

Abstract 

Using lactose as an inducer, recombinant human interleukin-2 (rhIL-2) was synthesized with an N-terminus fusion partner, G3 (three tandem-arranged glucagon peptides) in fed-batch cultures at high cell concentration (60-90 g l-1) of Escherichia coli BL21(DE3) [pT7-G3IL2]. With batch additions of lactose (4 x 13.5 g), the fusion rhIL-2 was synthesized up to 9.3 g l-1. However, if all the lactose (54 g) was added at once to the culture, synthesized fusion rhIL-2 decreased to 5.4 g l-1 with a decreased cell growth rate. A statistical optimization of the production medium containing glucose, yeast extract, and lactose led to fusion rhIL-2 being produced at > 9 g l-1.

ISSN 

0141-5492

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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