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Title 

Regulation of starch contents in potato (Solanum tuberosum L.) by manipulation of sucrose synthase gene

Authors 

Hyun Soon KimJae Heung JeonKyung Hwa ChoiYoung Hee JoungByoung Il LeeHyouk Joung

Issue Date 

2000

Citation 

Journal of Japanese Society Horticultural Science, vol. 69, no. 3, pp. 243-249

Keywords 

microtuberpatatin promoterstarch contentsucrose synthasetransgenic potato

Abstract 

Transgenic potato microtubers were created in which the expression of sucrose synthase (Susy) was altered by introducing a chimeric gene containing the coding region of Susy that is linked to the patatin promoter in sense (S) or antisense (AS) orientation. The analysis of transgenic microtubers showed that Susy activity and starch content increased in S plants 8, 22, and 30 transformed with the chimeric sense gene; they decreased in AS plants 3 and 10 transformed with the antisense gene. RT-PCR analysis revealed that the level of mRNA for Susy increased in S plants, and decreased in AS plants. There was a little change in the expression of genes for ADP-glucose pyrophosphorylase (AGPase), one of key enzymes in starch biosynthesis. The level of mRNA for AGPase and the activity of the enzyme increased in S 8 plant, but decreased in AS 3 plant.

ISSN 

0013-7626

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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