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Title | Anti-apoptotic effects of terrein on etoposide-induced apoptosis of U937 human leukemia cells |
| Terrein의 etoposide에 의해 유도된 apoptosis 저해효과 |
Authors | Choong Hwan Lee; Ho Jae Lee; Jin Hee Kim; Hyun A Kim; Yung Hee Kho |
Publisher | Korean Journal of Applied Microbiology and Biotechnology |
Issue Date | 2000 |
Citation | Korean Journal of Applied Microbiology & Biotechnology, vol. 28, no. 2, pp. 87-91 |
Keywords | apoptosis; inhibitor; terrein; etoposide; U937 cells |
Abstract | In the course of screening for the substances inhibiting apoptosis of U937 human leukemia cell induced by etoposide, a fungal strain F80834 producing a high level of inhibitor was selected. The inhibitory substance was purified and identified as terrein by spectroscopic methods of UV, EI-MS, 1H-NMR, 13C-NMR and DEPT. Terrein showed inhibitory activity of caspase 3, a major protease of apoptosis cascade, with an IC50 value of 20 μg/ml after 7 hrs of treatment. It also showed protective effect against cell death with an IC50 value of 10 μg/ml on U937 cells induced by etoposide after 24 hrs of treatment, but did not show any cytotoxicity at the same condition without etoposide. |
ISSN | 0257-2389 |
Appears in Collections | |
Registered Date |
2019-05-02 |
