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Title 

Enhancement of extracellular glucose oxidase production in pH-stat feed-back controlled fed-batch culture of recombinant Saccharomyces cerevisiae

Authors 

Arnab KapatJoon Ki JungYoung Hoon Park

Publisher 

Springer Verlag (Germany)

Issue Date 

1998

Citation 

Biotechnology Letters, vol. 20, no. 7, pp. 683-686

Keywords 

glucose oxidasefungus culturepHSaccharomyces cerevisiae

Abstract 

Glucose oxidase (GOD) from Aspergillus niger was expressed in Saccharomyces cerevisiae under the regime of GAL-10 promoter and GAL-7 terminator of S. cerevisiae and α-amylase signal sequence of Aspergillus oryzae. The enhancement of the expression level was achieved in pH-stat feed-back controlled fed-batch culture. The highest titre of extracellular GOD was 199 U/ml which marked two fold improvement over the batch (95 U/ml) and 28% above that of non-feed back controlled fed-batch (154 U/ml) operation.

ISSN 

0141-5492

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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