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Title 

Cloning and sequence analysis of another Shiga-like toxin IIe variant gene (slt-IIera) from an Escherichia coli R107 strain isolated from rabbit

Authors 

Sang Hyun KimIn Ho ChaKyoung Sook KimYong Hwan KimYoung Choon Lee

Publisher 

Wiley-Blackwell

Issue Date 

1997

Citation 

Microbiology and Immunology, vol. 41, no. 10, pp. 805-808

Keywords 

Shiga toxin genePathogenesis

Abstract 

An Escherichia coli R107 strain (O26 serotype) producing a Shiga-like toxin IIe variant (SLT-IIera) was isolated fram the mesenteric lymph node of a freshly dead rabbit carcass. The entire structural gene for this SLT-IIera was cloned from chromosomal DNA by PCR using primers based on previously published slt-IIe sequences. Nucleotide sequence analysis indicated that the slt-IIera gene was very similar to slt-IIe (formerly called slt-IIv) from E. coli strains S1191 and 412; five and one nucleotide changes were detected in A and B subunits, respectively, which resulted in changes in amino acid sequences of the corresponding subunits by three and one residues. Recombinant SLT-IIera and SLT-IIe produced using an E. coli host-vector system showed similar cytotoxicity, suggesting that the variations in the structural gene of SLT-IIera have no significant effect on cytotoxic level.

ISSN 

0385-5600

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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