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Title 

Effect of continuous feeding of galactose on the production of recombinant glucose oxidase using Saccharomyces cerevisiae

Authors 

Arnab KapatJoon Ki JungYoung Hoon Park

Publisher 

Springer

Issue Date 

2000

Citation 

Bioprocess Engineering, vol. 23, no. 1, pp. 37-40

Keywords 

galactoseglucose oxidaserecombinant enzymeSaccharomyces cerevisiae

Abstract 

A recombinant strain of Saccharomyces cerevisiae harboring GOD gene originated from Aspergillus niger was used for the production of extracellular glucose oxidase. The effect of continuous galactose feeding on the induction of GAL-10 promoter was examined in a 5 l bioreactor. The highest enzyme production level (164 U cm-3) was achieved at 96 h of cultivation. The production performance was compared with the results of fed-batch cultivations carried out in the same laboratory. Continuous feeding mode was found to be less productive due to excess ethanol formation and plasmid instability.

ISSN 

0178-515X

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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