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Title 

Analysis of production of thermostable alkaline protease using thermoactinomyces sp. E79

 

Thermoactinomyces sp. E79를 이용한 내열성 Alkaline 단백질 분해효소 생산 : 환경인자의 영향

Authors 

Sang Won JungSung Sik ParkYong Cheol ParkTae Kwang OhJin Ho Seo

Publisher 

Korean Journal of Applied Microbiology and Biotechnology

Issue Date 

2000

Citation 

Korean Journal of Applied Microbiology & Biotechnology, vol. 28, no. 3, pp. 167-171

Keywords 

Thermoactinomyces sp.Thermostable alkaline proteasealkaline proteinaseThermoactinomyces

Abstract 

This research was undertaken to analyze fermentation properties of Thermoactinomyces sp. E79 for production of a thermostable alkaline protease, which is able to specifically hydrolyze defatted soybean meal (DSM) to amino acids. The optimum pH for cell growth and protease production was pH 6.7. Thermoactinomyces sp. E79 did not grow at pH10 Among carbon sources tested, soluble starch was the best for protease production, while glucose repressed protease production. Tryptone was found to be the best nitrogen source for cell growth and soytone was good for protease production. Oxygen transfer rate played an important role in producing thermostable alkaline protease. Maximum values of 6.58 g/L of dry cell weight and 43.0 U/mL of protease activity were obtained in a batch fermentation using a 2.5 L jar fermentor at 1.93 x 102 hr-1 of volumetric oxygen transfer coefficient (κ(L)a). Addition of 200 mg/L humic acid to the growth medium resulted in 1.64 times higher protease activity and 1.77 times higher cell growth than the case without humic acid addition.

ISSN 

0257-2389

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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