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Title 

Time dependency of the action of nitric oxide in lipopolysaccharide-interferon-γ-induced neuronal cell death in murine primary neuron-glia co-cultures

Authors 

G H JeohnWon Gon KimJ S Hong

Publisher 

Elsevier

Issue Date 

2000

Citation 

Brain Research, vol. 880, no. 1, pp. 173-177

Keywords 

nitric oxidelipopolysaccharideneuronsglianeurotoxicityprimary cell culturecytokines

Abstract 

We investigated the time-dependency of the action of nitric oxide (NO) on glia-mediated neuronal cell death. Cortical neuron-glia co-cultures were treated with lipopolysaccharide and interferon γ (LPS/IFNγ). The production of NO was first detectable 9 h after the exposure to LPS/IFNγ and increased for up to 48 h. A significant neuronal cell death was observed 36-48 h after treatment with LPS/IFNγ. The NO generated at the initial stage of NO synthesis (about 12 h) following exposure to LPS/IFNγ was found to be critical for LPS/IFNγ-induced neurotoxicity. Furthermore, the rate of NO production at the initial stage of NO synthesis was correlated linearly with the extent of neuronal cell death. These findings suggest that the maximal rate of NO synthesis, instead of the accumulated NO2- level, is a sensitive index for predicting endotoxin-induced cytotoxicity.

ISSN 

0006-8993

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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