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Title 

Interaction of PRK1 receptor-like kinase with a putative elF2B β-subunit in tobacco

Authors 

Seong Whan ParkSung Hoon YuMoon Il KimSeung Cheol OhTeh Hui KaoHyun Sook Pai

Publisher 

Springer Verlag (Germany)

Issue Date 

2000

Citation 

Molecules and Cells, vol. 10, no. 6, pp. 626-632

Keywords 

βsubunit of eukaryotic translation factor 2Bin vitro protein binding assayPRK1 receptor-like kinaseyeast two hybrid screening

Abstract 

PRK1, a receptor-like kinase that is expressed in pollen, pollen tubes, and ovaries, has been shown to play important roles in pollen development and embryo sac development in Petunia inflata. We have used the kinase domain of PRK1 as a bait in the yeast two-hybrid system to identify PRK1-interacting proteins. The screening resulted in isolation of a cDNA encoding a protein highly homologous to the human and yeast beta-subunit of translation initiation factor 2B (eIF2B-beta), which was designated NeIF2Bbeta. eIF2B is a guanine nucleotide exchange protein that functions in the regulation of translation in eukaryotic cells. Deletion mutants of NeIF2Bbeta were analyzed for their interaction with PRK1, and the results suggested that the N-terminal half of NeIF2Bbeta, especially the region between residue 103 and 235, is important for the interaction. This protein association was confirmed by in vitro binding assay of the recombinant NeIF2Bbeta and PRK1 proteins. Despite high sequence homology between NeIF2Bbeta and its yeast counterpart, the NeIF2Bbeta cDNA could not rescue the phenotype of the yeast mutant strain lacking the GCD7 gene encoding eIF2B-beta, when transferred into the mutant strain.

ISSN 

1016-8478

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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