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Title 

High level expression of human lipocortin(annexin) 1 in Escherichia coli

Authors 

Kwang Lae HoeEun Hee ChoSoo Ok LeeDoe Sun Na

Publisher 

Springer Verlag (Germany)

Issue Date 

1996

Citation 

Biotechnology Letters, vol. 18, no. 2, pp. 163-168

Keywords 

lipocortin 1escherichia coliexpression vector

Abstract 

Human lipocortin (annexin) 1, a member of the annexin family of phospholipid binding proteins, has previously been expressed in E. coli (Huh et al., 1990). To improve the expression level of lipocortin 1 in E. coli, several expression vectors containing either the PL or the Ptrc promoter were constructed. The highest expression level, up to 20% of the total E. coli proteins, was obtained with plasmid pHT3. Plasmid pHT3 contains the pUC origin, the lipocortin 1 cDNA under Ptrc promoter, and the locI gene.

ISSN 

0141-5492

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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