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Title 

Overproduction of AgfA subunit of Salmonella enteritidis as a MBP-fusion protein in E. coli

Authors 

Mi Sun WonSo Youn KimSeung Hwan LeeChul Jung KimHyun Su KimMoo Hyung JunKyung Bin Song

Publisher 

Springer Verlag (Germany)

Issue Date 

2000

Citation 

Biotechnology Letters, vol. 22, no. 14, pp. 1165-1167

Keywords 

MBP-fusion proteinSalmonella entiritidisSEF 17

Abstract 

To study the characteristics of recombinant thin aggregative fimbriae of salmonella and to develop a vaccine for salmonella infections, the AgfA subunit gene was amplified from Salmonella entiritidis using PCR. Maltose binding protein (MBP)-AgfA fusion protein was over-produced in E. coli and purified. Antibody against MBP-AgfA was prepared and its immunogenicity was studied.

ISSN 

0141-5492

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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