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Title 

Effects of agitation and aeration on the production of extracellular glucose oxidase from a recombinant Saccharomyces cerevisiae

Authors 

Arnab KapatJoon Ki JungYoung Hoon ParkSung Yong HongHee Kyung Choi

Publisher 

Springer

Issue Date 

1998

Citation 

Bioprocess Engineering, vol. 18, no. 5, pp. 347-351

Keywords 

glucose oxidaseaerationagitationarticlenonhumanpriority journalsaccharomyces cerevisiaeSaccharomyces cerevisiae

Abstract 

A recombinant strain of Saccharomyces cerevisiae, GAL-GO2, was developed to facilitate the production of extracellular glucose oxidase (GOD). The recombinant strain secreted 85% (8.7 U/ml) of the total GOD (10.3 U/ml) produced in shake flask culture. For further enhancement of GOD production, optimization of the speed of agitation and the rate of aeration in a stirred tank fermentor was carried out. Response surface methodology with appropriate statistical experimental design was employed for this purpose. The maximal level of extracellular GOD was achieved when the speed of agitation and the rate of aeration were 420 rpm and 0.25 vvm, respectively. The enzyme production was increased by 74% compared to the level obtained under unoptimized conditions.

ISSN 

0178-515X

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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