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Title 

Characterization of an elastase inhibitor produced by Streptomyces lavendulae SMF11

Authors 

Hyun Sook LeeWook JinSung Gyun KangYoon Sook HwangYung Hee KhoKye Joon Lee

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2000

Citation 

Journal of Microbiology and Biotechnology, vol. 10, no. 1, pp. 81-85

Keywords 

elastaseinhibitordiketopiperazineStreptomyces lavendulaecompetitive inhibition

Abstract 

An elastase inhibitor, SMFEI02, was isolated from culture broth of Streptomyces lavendulae SMF11. The inhibitor was purified by ultrafiltration followed by XAD-7 column and Dowex-1 anion-exchange chromatographies, and preparative HPLC. The molecular formula was determined to be C14H16N2O2 (MW 244) by HRFAB-MS analysis. The inhibitor was identified to be a diketopiperazine cyclo(S-Phe-S-Pro) by the optical rotation value and NMR spectral data, and showed inhibitory activities for trypsin, chymotrypsin, cathepsin B, and papain as well as elastase with the K(i) values ranging from 1.78 mM to 2.86 μM. The inhibition showed a competitive mode for elastase, chymotrypsin, and cathepsin B, whereas it showed a noncompetitive mode for trypsin and papain.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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