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Title 

Prevention of encephalomyocarditis virus-induced diabetes by live recombinant Mycobacterium bovis bacillus Calmette-Guerin in susceptible mice

Authors 

Beom Kyu ChoiSang Hyun ChoGill Han BaiSang Jae KimByung Hwa HyunYong Kyung ChoeYong Soo Bae

Publisher 

American Diabetes Association

Issue Date 

2000

Citation 

Diabetes, vol. 49, no. 9, pp. 1459-1467

Keywords 

recombinant vaccinediabetes mellitusencephalomyocarditis virusmousemycobacterium bovisvirus infectioncardiovirus infectionsdiabetes mellitus, type 1micerecombinant proteins

Abstract 

The D variant of encephalomyocarditis (EMC-D) virus causes diabetes in susceptible mice by direct cytolysis of pancreatic β-cells. cDNA covering the major outer capsid protein (VP1) of the EMC-D virus was cloned into Mycobacterium bovis bacillus Calmette-Guerin (BCG). None of the SJL/J mice immunized with live recombinant BCG-VP1 (rBCG-VP1) became diabetic when challenged with the highly diabetogenic EMC-D virus, but the control mice inoculated with normal BCG developed diabetes during the same challenge. VP1-specific antibodies (including neutralizing antibodies) were markedly increased over time and reached the maximum titer at week 10 after a single immunization. The plateau of the titer lasted longer than 4 weeks. Mice and guinea pigs immunized with live rBCG-VP1 showed strong delayed-type hypersensitivity to the VP1 of the EMC-D virus. The preventive immunity still worked effectively 10 months after the primary immunization. At that time, the VP1-specific antibody was almost undetectable in the bloodstream, but a large number of VP1-specific lymphocytes was found in the spleen of the immunized mice. Our results show that live rBCG-VP1 elicits effective humoral and long-lasting cellular immune responses against EMC-D virus infection that results in the prevention of virus-induced diabetes in susceptible mice.

ISSN 

0012-1797

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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