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Title 

Surface-displayed viral antigens on Salmonella carrier vaccine

Authors 

Jong Soo LeeKwang Soon ShinJae Gu PanChul Joong Kim

Publisher 

Nature Publishing Group

Issue Date 

2000

Citation 

Nature Biotechnology, vol. 18, no. 6, pp. 645-648

Keywords 

Multivalent mucosal vaccineSalmonellaSurface displaylive vaccinerecombinant vaccinesalmonellosis vaccinevirus antigenantigen expressionAntigens, ViralBacterial Vaccines

Abstract 

We have developed a recombinant live oral vaccine using the ice- nucleation protein (Inp) from Pseudomonas syringae to display viral antigens on the surface of Salmonella spp. Fusion proteins containing viral antigens were expressed in the oral vaccine strain, Salmonella typhi Ty21a. Surface localization was verified by immunoblotting and fluorescence-activated cell sorting. The immunogenicity of surface-displayed viral antigens on the recombinant live vaccine strain was assessed in mice inoculated intranasally and intraperitoneally. Inoculation resulted in significantly higher serum antibody level than those induced by viral antigens expressed intracellularly. Thus, this multivalent mucosal live vaccine may provide an effective means for inducing mucosal or systemic immune responses against multiple viral antigens.

ISSN 

1087-0156

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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