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Title 

synthesis of cefminox by cell-free extracts of Streptomyces clavuligerus

Authors 

Jeong Keun KimHeui Il KangJeong Seok ChaeYoung Hoon ParkYong Jin Choi

Publisher 

Wiley-Blackwell

Issue Date 

2000

Citation 

FEMS Microbiology Letters, vol. 182, no. 2, pp. 313-317

Keywords 

7-Methoxylationcefminoxcell-free extractcephamycinproteus vulgaris GN 76/C-1streptomyces clavuligerus7alpha demethoxycefminoxcell free systemdrug synthesis

Abstract 

In vitro synthesis of cefminox by cell-free extracts of Streptomyces clavuligerus was investigated using α-ketoglutarate, L-ascorbic acid, FeSO4, S-adenosyl-L-methionine, and 7α-demethoxycefminox as the substrates. The formation of cefminox was detected both by a biological assay with Proteus vulgaris GN 76/C-1 and by high performance liquid chromatography. Although the conversion rate of 7α-demethoxycefminox to cefminox was observed to be quite low, it still demonstrated the potential for an enzymatic process to replace the chemical steps which are currently in use for the production of cefminox.

ISSN 

0378-1097

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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