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Title 

Comparative sequence analyses of the ribonuclease P (RNase P) RNA genes from LL-2,6-diaminopimelic acid-containing actinomycetes

Authors 

Jung Hoon YoonYong Ha Park

Publisher 

Society for General Microbiology

Issue Date 

2000

Citation 

International Journal of Systematic and Evolutionary Microbiology, vol. 50, no. 6, pp. 2021-2029

Keywords 

actinomycetesLL-2,6-diaminopimelic acidphylogenyRNase P RNA genediaminopimelic acidribonuclease Pgene sequencenucleotide sequencepolymerase chain reaction

Abstract 

Ribonuclease P (RNase P) RNA genes of LL-2,6-diaminopimelic acid (LL-A2pm)-containing actinomycetes, except Streptomyces species, were sequenced after PCR-amplification and cloning. By using the sequence data, the relationships between species within genera and the relationships between taxa above genus level were investigated and the usefulness of the RNase P RNA gene as another phylogenetic marker was evaluated. RNase P RNA gene sequences of all strains used in this study contained relatively conserved regions along with highly variable regions. The mean RNase P RNA gene similarity value was approximately 82±18% and the mean RNase P RNA gene similarity value when gaps were included was approximately 76±24%. The nucleotide similarities between the RNase P RNA genes of different strains were mostly fewer than the 16S rDNA similarities. The RNase P RNA gene was more useful than 16S rDNA for clearly differentiating the relationships between species belonging to a genus and the relationships between some genera. However, nucleotide sequences of RNase P RNA genes were not necessarily appropriate for comparisons at all taxonomic levels (such as those between species, between genera and between families).

ISSN 

1466-5026

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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