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Title 

Effects of Metal Ions on the Activity of Protein Tyrosine Phosphatase VHR: Highly Potent and Reversible Oxidative Inactivation by Cu2+ Ion

Authors 

Jin Hahn KimHyeong Jin ChoSeong Eon RyuMyung Un Choi

Publisher 

Elsevier

Issue Date 

2000

Citation 

Archives of Biochemistry and Biophysics, vol. 382, no. 1, pp. 72-80

Keywords 

copper ionmetal ionsoxidative inactivationprotein tyrosine phosphataseVHRphosphotyrosinechemical reactiongene overexpressionoxidation reduction reaction

Abstract 

The posttranslational regulation of protein tyrosine phosphatases (PTPs) has been suggested to have a crucial role in maintaining the phosphotyrosine level in cells. Here we examined the regulatory effects of metal ions on human dual-specificity vaccinia H1-related protein tyrosine phosphatase (VHR) in vitro. Among various metal ions examined, Fe3+, Cu2+, Zn2+, and Cd2+ exerted their inactivational effects on VHR, and Cu2+ is the most potent inactivator. The VHR activity inactivated by the metal ions except Cu2+ was significantly restored by EDTA. The efficacy of Cu2+ for the VHR inactivation was about 200-fold more potent than that of H2O2. Cu2+ also inactivated other PTPs including PTPIB and SHP-1. The Cu2+-mediated inactivation at the submicromolar range was eradicated by dithiothreitol treatment. The loss of VHR activity correlated with the decreased [14C]iodoacetate labeling of active-site cysteine, suggesting that Cu2+ brought about the oxidation of the active-site cysteine. On the contrary, Zn2+ that exerted an inactivational effect at millimolar concentrations appeared not directly linked to the active-site cysteine, as indicated by the fact that [14C]iodoacetate labeling was unaffected and that the effect of Zn2+ on the Y78F mutant was increased. The reduction potential of VHR was estimated to be -331 mV by utilizing the reversibility of the redox state of VHR. Thus, we conclude that the highly potent Cu2+ inactivation of VHR is a consequence of the oxidation of the active-site cysteine and the mode of Zn2+ inactivation is distinct from that of Cu2+.

ISSN 

0096-9621

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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