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Title 

Antimicrobial Peptide of Korean Native Goat Lactoferrin and Identification of the Part Essential for This Activity

Authors 

Michico KimuraMyoung Soo NamYoshifumi OhkouchiHaruto KumuraKei-ichi ShimazakiDae Yeul Yu

Publisher 

Elsevier

Issue Date 

2000

Citation 

Biochemical and Biophysical Research Communications, vol. 268, no. 2, pp. 333-336

Keywords 

lactoferrinpeptideantimicrobial activitygoatstructure activity relationgoatspeptide fragments

Abstract 

The antimicrobial activity of lactoferrin isolated from Korean native goat (KN goat) milk was studied and its antimicrobial domain was identified using synthetic peptides. Antimicrobial activity was assayed by a micro-method using 96-well microplates and a microplate reader. The amino acid sequence of the antimicrobial domain was suggested to be YQWQRRMRKLGAPSIT and this sequence corresponds to amino acid residues 20 to 35 of KN goat lactoferrin. Five peptides with certain amino acid residues deleted were synthesized in an effort to identify the residues essential for antimicrobial activity and it was found that the part with the sequence RRMRK (24-28) is the region most important for this activity. On the other hand, the conformation of the peptides did not influence the antimicrobial activity.

ISSN 

0006-291X

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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