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Title 

Receptor-mediated endocytosis of hepatitis B virus preS1 protein in EBV-transformed B-cell line

Authors 

Jung Hyun ParkEun Wie ChoDong Gun LeeJung Min ParkYun Jung LeeEun A ChoiKil Lyong Kim

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2000

Citation 

Journal of Microbiology and Biotechnology, vol. 10, no. 6, pp. 844-850

Keywords 

B-lymphocytesconfocal laser scanning microscopyepstein barr virushepatitis B virusinfectionpreS regionreceptorvirus DNAvirus proteinB lymphocyte

Abstract 

The specific binding and internalization of viral particles is an essential step for the successful infection of viral pathogens. In the case of the hepatitis B virus (HBV), virions bind to the host cell via the preS domain of the viral surface antigen and are subsequently internalized by endocytosis. HBV-preS specific receptors are primarily expressed on hepatocytes, however, viral DNA and proteins have also been detected in extrahepatic sites, suggesting that cellular receptors for HBV may also exist on extrahepatic cells. Recently, an EBV-transformed B-cell line was identified onto which the preS region binds in a receptor-ligand specific manner. In this study, this specific interaction was further characterized, and the binding region within the preS protein was localized. Also the internalization after host cell attachment was visualized and analyzed by fluorescence-labeled HBV-preS1 proteins using confocal microscopy. Energy depletion by sodium azide treatment effectively inhibited the internalization of the membrane-bound preS1 ligands, thereby indicating an energy-dependent receptor-mediated endocytotic pathway. Accordingly, the interaction of HBV-preS1 with this specific B-cell line may serve as an effective model for an infection pathway in extrahepatic cells.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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