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Title 

Crystallization and preliminary X-ray crystallographic analysis of NAD(+)-dependent DNA ligase from Thermus filiformis

Authors 

Jae Young LeeHyun Kyu KimChang Soo ChangSoo Hyun EomKwang Yeon HwangYun Je ChoYeon Gyu YuSeong Eon RyuSuk Tae KwonSe Won Suh

Publisher 

International Union of Crystallography

Issue Date 

2000

Citation 

Acta Crystallographica. Section D, Biological Crystallography, vol. 56, no. 3, pp. 357-358

Keywords 

polydeoxyribonucleotide synthasecrystallizationthermusX ray crystallographycrystallography, X-RayDNA LigasesNADthermus filiformis

Abstract 

A highly thermostable DNA ligase from Thermus filiformis has been crystallized at room temperature using methoxypolyethylene glycol 5000 as a precipitant. The crystal belongs to the monoclinic space group P21, with unit-cell parameters a = 90.63, b = 117.80, c = 98.65 ?, β = 115.56°. Two molecules of DNA ligase are present in the asymmetric unit, giving a crystal volume per protein mass (V(m)) of 3.1 ?3Da-1 and a solvent content of 61%. A native data set extending to 3.0 ? resolution has been collected at 100 K using synchrotron X-rays.

Citation 

Acta Crystallographica. Section D, Biological Crystallography, 56(3): 357-358

ISSN 

0907-4449

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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