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Title 

Activation of mitogen-activated protein kinase pathways by angelan in murine macrophages

Authors 

Young Jin JeonSang Bae HanSang Han LeeHyoung Chin KimKyung Seop AhnHwan Mook Kim

Publisher 

Elsevier

Issue Date 

2001

Citation 

International Immunopharmacology, vol. 1, no. 2, pp. 237-245

Keywords 

angelica gigas Nakaiangelanmacrophagesp38PTK

Abstract 

In our previous studies, we showed that angelan, a polysaccharide purified from Angelica gigas Nakai, specifically activated macrophages to induce cytokines including inducible nitric oxide synthase (iNOS) which has strong anti-tumor activities [Immunopharmacology, 1999; 43: 1.]. In the present study, we investigated the intracellular signal transduction pathways involved in the angelan-induced iNOS synthesis by murine macrophages. Protein tyrosine phosphorylation was induced within 5 min by angelan, and the blocking of protein tyrosine kinases (PTKs) inhibited down-stream pathways leading to iNOS production in response to angelan. Treament of RAW 264.7 cells with angelan resulted in significant activation of extracellular signal-regulated kinase 1 and 2 (ERK1/2) and p38, while stress-activated protein kinase/c-Jun NH2 terminal kinase (SAPK/JNK) was not activated by angelan. The specific p38 inhibitor SB203580 abrogated the angelan-induced iNOS synthesis, whereas the selective mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase 1 (MEK-1) inhibitor PD98059 did not affect the iNOS induction. In conclusion, we demonstrate that PTK and p38 MAPK activation are required to transduce signals leading to iNOS expression in angelan-stimulated murine macrophages.

ISSN 

1567-5769

Link 

http://dx.doi.org/10.1016/S1567-5769(00)00003-5

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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