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Title 

Mutation of the homologue of GDP-mannose pyrophosphorylase alters cell wall structure, protein glycosylation and secretion in Hansenula polymorpha

Authors 

Michael O AgaphonovAnna N PackeiserMaria B ChechenovaEui Sung ChoiMichael D Ter-Avanesyan

Publisher 

Wiley-Blackwell

Issue Date 

2001

Citation 

Yeast, vol. 18, no. 5, pp. 391-402

Keywords 

cell wallhansenula polymorphaprotein glycosylationprotein secretionurinary type plasminogen activatoryeast

Abstract 

A Hansenula polymorpha mutant with enhanced ability to secrete a heterologous protein has been isolated. The mutation defines a gene, designated OPU24, which encodes a protein highly homologous to GDP-mannose pyrophosphorylase Psa1p/Srb1p/Vig9p of Saccharomyces cerevisiae and CaSrb1p of Candida albicans. The opu24 mutant manifests phenotypes similar to those of S. cerevisiae mutants depleted for GDP-mannose, such as cell wall fragility and defects in N- and O-glycosylation of secreted proteins. The influence of the opu24 mutation on endoplasmic reticulum-associated protein degradation is discussed. The GenBank Accession No. for the OPU24 sequence is AF234177.

ISSN 

0749-503X

Link 

http://dx.doi.org/10.1002/yea.678

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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