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Title 

Cloning and characterization of the Hansenula polymorpha homologue of the Saccharomyces cerevisiae MNN9 gene

Authors 

So Young KimJung Hoon SohnHyun Ah KangSeung Ku YooYu Ryang PyunEui Sung Choi

Publisher 

Wiley-Blackwell

Issue Date 

2001

Citation 

Yeast, vol. 18, no. 5, pp. 455-461

Keywords 

yeasthansenula polymorphaMNN9glycosylation

Abstract 

A gene homologous to Saccharomyces cerevisiae MNN9 has been cloned and characterized in the methylotrophic yeast Hansenula polymorpha. This gene was cloned from a H. polymorpha genomic DNA library using the S. cerevisiae MNN9 gene as a probe. The H. polymorpha MNN9 homologue (HpMNN9) contained a 1062 bp open reading frame encoding a predicted protein of 354 amino acids. The deduced amino acid sequence showed 58% and 51% identity, respectively, with the S. cerevisiae and Candida albicans Mnn9 proteins. Disruption of HpMNN9 leads to phenotypic effects suggestive of cell wall defects, including detergent sensitivity and hygromycin B sensitivity. The hygromycin B sensitivity of S. cerevisiae mnn9 null mutant was complemented in the presence of the HpMNN9 gene. The DNA sequence of the H. polymorpha homologue has been submitted to GenBank with the Accession No. AF264786.

ISSN 

0749-503X

Link 

http://dx.doi.org/10.1002/yea.699

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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