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Title 

Tissue Engineered Artificial Skin Composed of Dermis and Epidermis

Authors 

Eun Kyung YangYoung Kwon SeoHee Hun YounDoo Hoon LeeSue Nie ParkJung Keug Park

Publisher 

Wiley-Blackwell

Issue Date 

2000

Citation 

Artificial Organs, vol. 24, no. 1, pp. 7-17

Keywords 

artificial skincollagen gelcollagen meshfibroblastkeratinocytedermisepidermisskin graftskin, artificial

Abstract 

We made an artificial skin comprised of a stratified layer of keratinocytes and a dermal matrix with a type I collagen containing fibroblasts. In this work, we showed keratinocyte behavior under primary culture, gel contractions varying with concentration of collagen solution, and cell growth plots in the collagen gel. The optimum behavior of dermal equivalent could be obtained using 3.0 mg/ml collagen solution and attached gel culture. The attached gel culture had a jumping effect of growth factor on cell growth at the lag phase. To develop the artificial skin, 1 x 105 cells/cm2 of keratinocytes were cultured on the dermal equivalent at air- liquid interface. Finally, to overcome the problem that artificial skin of collagen gel was torn easily during suturing of grafting, we prepared histocompatible collagen mesh and attached the mesh to the bottom of the gel. Cultured artificial skins were successfully grafted onto rats.

ISSN 

0160-564X

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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