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Title 

Hepatic Ischemia/Reperfusion in Rats Induces iNOS Gene Transcription by Activation of NF-κB

Authors 

Gang Min HurYoung Sue RyuHyo Yung YunByeong Hwa JeonYong Man KimJeong Ho SeokJae Heun Lee

Publisher 

Elsevier

Issue Date 

1999

Citation 

Biochemical and Biophysical Research Communications, vol. 261, no. 3, pp. 917-922

Keywords 

immunoglobulin enhancer binding proteinenzyme activationgenetic transcriptionliver ischemiaprotein bindingratgene ExpressionischemiaNF-kappa Brats

Abstract 

It has been known that many immediately early genes are expressed during ischemia/reperfusion (I/R) injury. Here, employing a model of hepatic I/R, we show that inducible nitric oxide synthase (iNOS) is induced via the activation of nuclear factor kappaB (NF-κB) after I/R in rat liver. When liver was subjected to ischemia followed by reperfusion, but not ischemia alone, an NF-κB complex composed of p50/p65 heterodimer and p50 homodimer was rapidly activated within 1 h and remained elevated for up to 3 h, and then tended to decline after 5 h of reperfusion. Also, the expression of iNOS mRNA was initiated after 1 h and continued to increase after 5 h of reperfusion during the time course studied. This upregulated iNOS mRNA expression coincides with increased iNOS enzyme activity and NF-κB binding activity after hepatic I/R. Administration of N-acetylcysteine (NAC, 20 mg/kg i.v. 10 min before reperfusion), an antioxidant, not only significantly inhibited the expression of iNOS mRNA but also blocked upregulated NF-κB binding activity after reperfused liver. These results suggest that NF-κB is activated by oxidative stress during hepatic I/R and may play a significant role in the induction of the iNOS gene.

ISSN 

0006-291X

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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