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Title 

Benzothiadiazole enhances the elicitation of rosmarinic acid production in a suspension culture of Agastache rugosa O. Kuntze

Authors 

Hye Kyong KimSei Ryang OhHyeong Kyu LeeHoon Huh

Publisher 

Springer Verlag (Germany)

Issue Date 

2001

Citation 

Biotechnology Letters, vol. 23, no. 1, pp. 55-60

Keywords 

agastache rugosabenzothiadiazolerosmarinic acidsuspension cultureyeast elicitor

Abstract 

Callus and suspension cultures were established from the leaves of Agastache rugosa. The suspension cell growth was maximum at 15 days after inoculation. The cellular content of rosmarinic acid increased slowly and reached maximum (0.42 mg g-1 dry wt) during the stationary phase of culture, after 18 days of inoculation. The addition of yeast extract preparation (MW < 10000) at 50 μg ml-1 elevated the rosmarinic acid content up to 5.7-fold of that found in non-elicited suspension cells. The elicitation of yeast extract preparation was further 2-fold enhanced by the presence of benzothiadiazole, a synthetic activator of plant systemic acquired resistance, as compared to yeast extract alone. These results showed that benzothiadiazole can be used as a tool for enhancing secondary metabolite accumulation in cell cultures.

ISSN 

0141-5492

Link 

http://dx.doi.org/10.1023/A:1026738409671

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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