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Title 

Enhanced biosynthesis of P(3HB-3HV) and P(3HB-4HB) by amplification of.the cloned PHB biosynthesis genes in Alcaligenes eutrophus

Authors 

Yong Hyun LeeJin Seo ParkTae Lin Huh

Publisher 

Springer Verlag (Germany)

Issue Date 

1997

Citation 

Biotechnology Letters, vol. 19, no. 8, pp. 771-774

Keywords 

propionic acidbiosynthesisgene amplificationwautersia eutrophaalcaligenes

Abstract 

The biosynthesis of P(3HB-3HV) and P(3HB-4HB) was carried out using transformants of Alcaligenes eutrophus harboring the cloned phbCAB, phbAB, and phbC genes. The molar fractions and yields of 3HV and 4HB increased significantly by enhancing enzymes related to PHB biosynthesis compared to the parent strain. Especially, PHB synthase was the most critical enzyme that regulated monomer compositions of P(3HB-3HV) and P(3HB-4HB) in the transformant. Even at the lower propionate or 4-hydroxybutyrate concentrations, the high molar fractions of 3HV or 4HB could be accumulated. The enforcement of PHB biosynthetic enzymes through the transformation of corresponding genes was identified to be an excellent method for modification of monomer composition of copolymer of A. eutrophus.

ISSN 

0141-5492

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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