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Title 

Effect of cross-linking agents on L-sorbose production by immobilized Gluconobacter suboxydans cells

Authors 

Young Min ParkSang Ki RheeEui Sung ChoiIn Sik Chung

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

1998

Citation 

Journal of Microbiology and Biotechnology, vol. 8, no. 6, pp. 696-699

Keywords 

cross-linking agentsD-sorbitolglutaraldehydeL- sorbosepolyethyleneiminegluconic acidsorbitolsorbosecell membrane permeability

Abstract 

Biological oxidation of D-sorbitol to L-sorbose using permeated and immobilized cells of Gluconobacter suboxydans was carded out to investigate the optimum reaction condition. The stabilization effect of cross-linking agents such as glutaraldehyde, tannic acid, and polyethyleneimine to prevent the leakage of enzymes from beads containing permeated and immobilized cells of G. suboxydans was examined by the production of L-sorbose from the mixture of D-sorbitol and gluconic acid. The protein concentration effused from immobilized beads treated with only glutaraldehyde was 5.2 μg/ml after 20 h. The beads of G. suboxydans immobilized with alginate and cross-linked with 0.3% glutaraldehyde was the most useful for the oxidation of D-sorbitol to L- sorbose.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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