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Title 

Comparison of characteristics of levan produced by different preparations of levansucrase from Zymomonas mobilis

Authors 

Ki Hyo JangKi Bang SongChul Ho KimBong Hyun ChungSoon Ah KangUck Han ChunRyo Won ChoueSang Ki Rhee

Publisher 

Springer Verlag (Germany)

Issue Date 

2001

Citation 

Biotechnology Letters, vol. 23, no. 5, pp. 339-344

Keywords 

immobilizationlevansucraselow molecular weight levanpermeabilization

Abstract 

The characteristics of levan formation by different preparations of levansucrase (free and immobilized enzyme and toluene-permeabilized whole cells), derived from recombinant levansucrase from Zymomonas mobilis expressed in Escherichia coli, were investigated. The maximal yield of levan by the three preparations were similar and were about 70-80% on a fructose-released basis with sucrose as nutrient at 100 g l-1. Immobilized enzyme and toluene-permeabilized whole cells produced low molecular weight levan (2-3 × 106), as determined by HPLC while high molecular weight levan (>6 × 106) was the major product with the free levansucrase. The size of levan can thus be controlled by immobilized levansucrase and toluene-permeabilized whole cells in high yield.

ISSN 

0141-5492

Link 

http://dx.doi.org/10.1023/A:1005641220946

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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