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Title 

Involvement of acetyl phosphate in the in vivo activation of the response regulator ComA in Bacillus subtilis

Authors 

Seong Bin KimByung Sik ShinSoo Keun ChoiChi Kyung KimSeung Hwan Park

Publisher 

Wiley-Blackwell

Issue Date 

2001

Citation 

FEMS Microbiology Letters, vol. 195, no. 2, pp. 179-183

Keywords 

two-component signal transductionComAacetyl phosphatesrfA-lacZbacillus subtilis

Abstract 

Development of genetic competence in Bacillus subtilis is regulated by ComP-ComA, a two-component signal transduction system. The response regulator ComA is primarily activated by ComP, a histidine kinase that mediates response to nutrient conditions and cell density, and the activated ComA is required for transcription of the srf operon, which is essential for the development of genetic competence and surfactin production. In this study we suggested that the ComA could also be activated by a small molecule phospho-donor, acetyl phosphate. Examination of srfA-lacZ expression indicated that a significant amount of srfA expression still occurs in the comP mutant during growth in a sporulation medium containing excess glucose. Analysis of a comP and pta mutant suggests that srfA activation seen in the comP mutant is dependent on the expression of pta, which encodes phosphotransacetylase (Pta). As Pta is responsible for the catalysis for conversion of acetyl coenzyme A to acetyl phosphate, we conclude that the expression of srfA seen in the comP mutant is mainly due to the activation of ComA by acetyl phosphate.

ISSN 

0378-1097

Link 

http://dx.doi.org/10.1016/S0378-1097(01)00008-8

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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