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Title 

Translocation and accumulation of exogeneous hepatitis B virus preS surface proteins in the cell nucleus

Authors 

Eun Wie ChoJung Hyun ParkOok Joon YooKil Lyong Kim

Publisher 

Company of Biologists

Issue Date 

2001

Citation 

Journal of Cell Science, vol. 114, no. 6, pp. 1115-1123

Keywords 

hepatitis B virusPreS2 regionenergy-independent translocation

Abstract 

Recurrent reports about protease-sensitive sites in the junction of the preS and S region of the hepatitis B virus large surface protein have raised the question about a possible biological role of S protein-depleted, independent preS protein fragments in the virus life cycle. In the present study, this question was addressed by exogenous introduction of fluorescence-labeled recombinant preS proteins into permeabilized HepG2 cells. While maltose-binding proteins (MBP) were evenly distributed throughout the cytoplasm, MBP-preS fusion proteins selectively accumulated in the nucleus. Using truncated preS proteins, the effective domain for this nuclear accumulation was localized around the preS2 region. The mode of this action differs from conventional nuclear translocation mechanism in its energy- and mediator-independency and in that it is not saturated regardless of the increase of preS protein concentration. The biological meaning of this phenomenon has to be further studied. However, in regard to hepatitis B virus infection, this observation might provide a clue for unveiling the still poorly characterized events after initial internalization of the virus, which might make use of the nuclear translocation effect of the preS2 region to facilitate the infection.

ISSN 

0021-9533

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2018-08-01


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