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Title 

Enhanced transformation efficiency of an anticoagulant hirudin gene into Saccharomyces cerevisiae by a double δ-sequence

Authors 

Myoung Dong KimYoung Je YooSang Ki RheeJin Ho Seo

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2001

Citation 

Journal of Microbiology and Biotechnology, vol. 11, no. 1, pp. 61-64

Keywords 

Hirudinδ-intergrationSaccharomyces cerevisiaetransformation efficiency

Abstract 

Delta-integration vectors were constructed for the purpose of achieving homologous integration of the hirudin expression cassette into the chromosome of Saccharomyces cerevisiae. A double δ system truncated with the unnecessary bacterial genes, and consequently having a reduced insert size for integration, showed a four-fold increase in transformation efficiency at given DNA concentrations, and as a result, the constructed recombinant yeast strain had a 1.3-fold enhancement in hirudin expression level compared with a single δ system.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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