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Title 

Prediction of the secondary structure of the AgfA subunit of Salmonella enteritidis overexpressed as an MBP-fused protein

Authors 

Mi Sun WonSo Youn KimSeung Hwan LeeChul Jung KimHyun Su KimMoo Hyung JunKyung Bin Song

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2001

Citation 

Journal of Microbiology and Biotechnology, vol. 11, no. 1, pp. 164-166

Keywords 

secondary structuresalmonella enteritidisAgfA

Abstract 

To examine the characteristics of the recombinant thin aggregative fimbriae of Salmonella, the AgfA subunit gene was amplified from Salmonella enteritidis using a PCR. The maltose binding protein (MBP)-AgfA fusion protein was overproduced in E. coli and purified. The secondary structure of AgfA was then elucidated from the difference CD spectra. An estimation of the secondary structure of AgfA using the self-consistent method revealed a mostly β-sheet structure.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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