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Title 

Enhanced production of anticoagulant hirudin in recombinant Saccharomyces cereΩisiae by chromosomal δ-integration

Authors 

Myoung Dong KimSang Ki RheeJin Ho Seo

Publisher 

Elsevier

Issue Date 

2001

Citation 

Journal of Biotechnology, vol. 85, no. 1, pp. 41-48

Keywords 

hirudinSaccharomyces cerevisiaemitotic stabilityδ-integrationcopy number

Abstract 

Recombinant Saccharomyces cerevisiae strains were developed to overproduce an anticoagulant hirudin. The δ-sequences of the yeast retrotransposon Ty1 and URA3 were used as target sites for a hirudin expression cassette. High copy-number transformants were successfully selected using a dominant selection antibiotic, G418. The copy numbers of the hirudin expression cassette integrated into δ-sequences of the yeast chromosome ranged from five to ten copies per cell. Production of hirudin in the δ-integrated recombinant S. cerevisiae system increased over two-fold compared with the YEp-based episomal hirudin expression system. A linear relationship between the copy number of the hirudin expression cassette and hirudin expression level was observed up to 10 copies. The hirudin expression cassettes integrated into the yeast chromosome were stably maintained in non-selective culture conditions.

ISSN 

0168-1656

Link 

http://dx.doi.org/10.1016/S0168-1656(00)00376-X

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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