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Title 

Activation of the IGF-II Gene by HBV-X Protein Requires PKC and p44/p42 Map Kinase Signalings

Authors 

Suk Mi Kang-ParkJe Ho LeeJeh Hoon ShinYoung Ik Lee

Publisher 

Elsevier

Issue Date 

2001

Citation 

Biochemical and Biophysical Research Communications, vol. 283, no. 2, pp. 303-307

Keywords 

HBV-Xhepatocellular carcinomasIGF-IISp1phosphorylationPKCMAPK

Abstract 

We have recently shown that HBx protein, one of the causative agents of hepatocellular carcinomas, regulates Sp1 mediated transcription of insulin-like growth factor II promoter 4 (Lee et al. (1998) Oncogene 16, 2367-2380). Here we show that PKC and p44/p42MAPK signalings are required for the HBx-induced Sp1-mediated IGF-II P4 transcriptional activity since (i) PKC activation by PMA or PKC expression vector increases Sp1 phosphorylation and P4 activity in HBx-transfected HepG2 cells; (ii) PKC inhibition by PKC inhibitor G?6976 reduces Spl phosphorylation, P4 activity, and IGF-II mRNA in HBx-transfected HepG2 cells; and (iii) the inhibition of MEK activation by U0126 reduces Sp1 phosphorylation, P4 activity and IGF-II mRNA in HBx-transfected HepG2 cells. These results demonstrate that PKC and p44/p42 MAPK cascades are the essential signaling pathways in Sp1-mediated IGF-II gene activation by HBx.

ISSN 

0006-291X

Link 

http://dx.doi.org/10.1006/bbrc.2001.4767

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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