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Title 

A novel dual-specificity protein kinase targeted to the chloroplast in tobacco

Authors 

Hye Sun ChoGyeong Mee YoonSang Sook LeeYoung A. KimIn Hwan HwangDo Il ChoiHyun Sook Pai

Publisher 

Elsevier

Issue Date 

2001

Citation 

FEBS Letters, vol. 497, no. 2, pp. 124-130

Keywords 

chloroplast-targeteddual-specificity protein kinaselight- and gibberellin-stimulated expressionNtDSK1tobacco

Abstract 

The NtDSK1 cDNA encoding a novel chloroplast-targeted protein kinase was identified in Nicotiana tabacum. It contains the kinase domain at the C-terminus and a putative regulatory domain at the N-terminus. The recombinant NtDSK1 underwent autophosphorylation of serine, threonine, and tyrosine residues, indicating that NtDSK1 encodes a functional dual-specificity protein kinase. The NtDSK1-green fluorescent protein fusion protein was targeted to chloroplasts. Furthermore, the NtDSK1 protein was immunodetected in chloroplast fractions isolated from tobacco seedlings. The NtDSK1 mRNA expression was developmentally regulated in different tissues, including anthers and germinating seeds, and strongly stimulated by gibberellin. The mRNA was rapidly light responsive during seedling growth. NtDSK1 may play a role in a light-regulated signaling process in tobacco.

ISSN 

0014-5793

Link 

http://dx.doi.org/10.1016/S0014-5793(01)02439-5

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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