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Title 

Molecular identification of IgE-dependent histamine-releasing factor as a B cell growth factor

Authors 

Hyung Sik KangMin Ju LeeHyun keun SongSeung Hyun HanYong Man KimJoo Young ImIn Pyo Choi

Publisher 

American Association of Immunologists

Issue Date 

2001

Citation 

Journal of Immunology, vol. 166, no. 11, pp. 6545-6554

Keywords 

bacterial DNACD40 antigengrowth factorhistamineimmunoglobulin Emajor histocompatibility antigen class 2neutralizing antibodyamino terminal sequenceantigen specificityB lymphocyte activation

Abstract 

The culture supernatants of LK1 cells, murine erythroleukemia cells, showed B cell-stimulating activity. Purification and NH2-terminal sequence analysis revealed that one of the candidates was murine IgE-dependent histamine-releasing factor (IgE-HRF), which is known to induce histamine from basophils. Recombinant IgE-HRF (rHRF) obtained from Escherichia coli- or 293-transformed embryonal kidney cells was tested for B cell-stimulating activity. Both rHRFs stimulated B cell proliferation in a dose-dependent manner. However, boiling or anti-HRF Ab abolished the B cell stimulatory effects of rHRF. Recombinant HRF showed strong synergistic effects with IL-2, IL-4, and IL-5 for B cell activation, with maximal activity in the presence of anti-CD40 Ab. Recombinant HRF increased MHC class II expression of B cells. It also increased Ig production from B cells. Treatment with polymyxin B, a neutralizing peptide antibiotic of LPS, did not reduce the activity of rHRF. In addition, FACS analysis using PE-conjugated rHRF showed that HRF bound to B cells. Recombinant HRF up-regulated the expression of IL-1 and IL-6 in B cells. In vivo administration of rHRF or the cDNA for rHRF increased total and Ag-specific Ig synthesis. Taken together, these results indicate that HRF stimulates B cell activation and function.

ISSN 

0022-1767

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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