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Title 

Influence of oocyte nuclei on demethylation of donor genome in cloned bovine embryos

Authors 

Yong Kook KangDeog Bon KooJung Sun ParkYoung Hee ChoiKyung Kwang LeeYong Mahn Han

Publisher 

Elsevier

Issue Date 

2001

Citation 

FEBS Letters, vol. 499, no. 1, pp. 55-58

Keywords 

epigeneticnuclear transferbisulfitereprogramming

Abstract 

We recently demonstrated that satellite regions exhibit an aberrant DNA methylation in cloned bovine embryos. Here, we examined, using bisulfite-sequencing technology, whether the inefficient demethylation of cloned donor genomes could be rescued by the presence of oocytic nuclei. Both AciI digestion and sequencing analyses showed that satellite sequence was demethylated more efficiently in cloned tetraploid blastocysts than in diploid clones. When methyl-CpG density (the number of methyl-CpG sites per string) was scored, a significant decrease was observed in tetraploids (P < 0.001). These results suggest that unknown mechanisms provided by oocytic nuclei could assist the demethylation of satellite sequences in tetraploid clones.

ISSN 

0014-5793

Link 

http://dx.doi.org/10.1016/S0014-5793(01)02514-5

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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