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Title 

Identification of Streptomyces sp. AMLK-335 producing antibiotic substance inhibitory to vancomycin-resistant enterococci

Authors 

Ki Hyeong RheeKyung Hee ChoiChang Jin KimChang Han Kim

Publisher 

The Korean Society for Applied Microbiology

Issue Date 

2001

Citation 

Journal of Microbiology and Biotechnology, vol. 11, no. 3, pp. 469-474

Keywords 

vancomycin-resistant enterococci (VRE)16S rDNAstreptomyces sp. AMLK-335cyclo (L-phenylalanyl-L-prolyl)

Abstract 

The actinomycete strain AMLK-335 was antagonistic to vancomycin-resistant enterococci (VRE). Based on the diaminopimelic acid (DAP) type, and morphological and physiological characteristics revealed by scanning electron microscopy (SEM), AMLK-335 was confirmed to belong to the genus Streptomyces. Analysis of the 16S rDNA nucleotide sequences found AMLK-335 to have a relationship with Streptomyces platensis. The production of antibiotic from this strain was most favorable when cultured on glucose, polypeptone, yeast extract (PY) medium for 6 days at 27 °C. The antibiotic was identified as cyclo(L-phenylalanyl-L-prolyl) by comparing it with the reported MS and NMR spectral data. Cyclo(phe-pro) from the PY cultures of AMLK-335 was most effective against the VRE strains E. faecium (K-98-637) and E. faecalis (K-98-258). Furthermore, cyclo(phe-pro) had antimicrobial activity against Bacillus subtilis, Microcuccs luteus, Staphylococcus aureus, and Saccharomyces cerevisiae, but it was ineffective against Candida albicans, Streptomyces murinus, and Aspergillus niger.

ISSN 

1017-7825

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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