상세 정보

underline
Metadata Downloads : dc(xml) or Excel
Cited 0 time in scopus ci

Title 

Enantioselective hydrolysis of racemic naproxen methyl ester by two-step acetone-treated Candida rugosa lipase

Authors 

Eun Gyo LeeHye Soon WonBong Hyun Chung

Publisher 

Elsevier

Issue Date 

2001

Citation 

Process Biochemistry, vol. 37, no. 3, pp. 293-298

Keywords 

candida rugosa lipase (CRL)enantioselective hydrolysis(S)-Naproxen(R,S)-Naproxen methyl estertwo-step acetone treatment

Abstract 

An optically pure (S)-naproxen was produced by two-step acetone-treated Candida rugosa lipase (CRL) through enantioselective hydrolysis of racemic naproxen methyl ester. The two-step acetone-treated CRL was much more enantioselective than the crude CRL towards the hydrolysis of (R,S)-naproxen methyl ester, yielding an enantiomeric excess (eep) of > 98% and an enantiomeric ratio (E) of > 100. In terms of hydrolysis reaction rate and enantioselectivity, the optimal reaction conditions were found to be 37 °C and pH 6.0. The scaled-up production of (S)-naproxen was performed in a batch reactor containing 200 ml of substrate solution (50 mM MES buffer containing 200 mM (R,S)-naproxen methyl ester). After 156 h of reaction, 38.4% of the initial (R,S)-naproxen methyl ester was hydrolyzed, yielding an enantiomeric excess of 98% and an enantiomeric ratio of > 100. Finally, (S)-naproxen was recovered from the reaction mixture with an optical purity of 98% and a recovery yield of 95%.

ISSN 

0032-9592

Link 

http://dx.doi.org/10.1016/S0032-9592(01)00213-8

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


There are no files associated with this item.
qrcode

FusionCharts.
DSpace Software Coptright(c) 2010 MIT and Hewleft-Packard  /  KRIBB-REPOSITORY ( Email:jakim@kribb.re.kr)