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Title 

Dendritic cell-tumor coculturing vaccine can induce antitumor immunity through both NK and CTL interaction

Authors 

Kwang Dong KimSeung Chul ChoiAe Yung KimYong Kyung ChoeIn Seong ChoeJong-Seok Lim

Publisher 

Elsevier

Issue Date 

2001

Citation 

International Immunopharmacology, vol. 1, no. 12, pp. 2117-2129

Keywords 

dendritic cells (DC)natural killer (NK) cellsCTLantitumor activityIFN-γ

Abstract 

Immunization of dendritic cells (DC) pulsed with tumor antigen can activate tumor-specific cytotoxic T lymphocytes (CTL) that are responsible for protection and regression. We show here that immunization with bone marrow-derived DC cocultured with tumor cells can induce a protective immunity against challenges to viable tumor cells. In this study, we further investigated the mechanism by which the antitumor activity was induced. Immunization of mice with DC cocultured with murine colon carcinoma, CT-26 cells, augmented CTL activity against the tumor cells. Concomitantly, an increase in natural killer (NK) cell activity was also detected in the same mice. When DC were fixed with paraformaldehyde prior to coculturing with tumor cells, most of the CTL and NK cell activity diminished, indicating that DC are involved in the process of presenting the tumor antigen(s) to CTL. NK cell depletion in vivo produced markedly low tumor-specific CTL activity responsible for tumor prevention. In addition, RT-PCR analysis confirmed the high expression of INF-γ mRNA in splenocytes after vaccination with DC cocultured with tumors, but low expression in splenocytes from NK-depleted mice. Most importantly, the tumor protective effect rendered to DC by the coculturing with CT-26 cells was not observed in NK-depleted mice, which suggests that DC can induce an antitumor immune response by enhancing NK cell-dependent CTL activation. Collectively, our results indicate that NK cells are required during the priming of cytotoxic T-cell response by DC-based tumor vaccine and seem to delineate a mechanism by which DC vaccine can provide the desired immunity.

ISSN 

1567-5769

Link 

http://dx.doi.org/10.1016/S1567-5769(01)00137-0

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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