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Title 

Effects of methanol on expression of an anticoagulant hirudin in recombinant Hansenula polymorpha

Authors 

M D KimKang Hyun AhSang Ki RheeJ H Seo

Publisher 

Springer

Issue Date 

2001

Citation 

Journal of Industrial Microbiology (& Biotechnology), vol. 27, no. 1, pp. 58-61

Keywords 

hansenula polymorphahirudinmethanolmethanol oxidase promoteranticoagulant therapyprotein expressionhirudins

Abstract 

A series of batch, fed-batch, and continuous cultures was carried out to analyze the effects of methanol on the fermentation characteristics of recombinant Hansenula polymorpha for the production of hirudin, an anticoagulant. Hirudin expression efficiencies were greatly influenced by the methanol concentrations in continuous and fed-batch culture modes. At a steady state of continuous culture, an optimum methanol concentration of 1.7 g I-1 was determined at a dilution rate of 0.18 h-1 with 1.8 mg I-1 h-1 hirudin productivity.

ISSN 

0169-4146

Link 

http://dx.doi.org/10.1038/sj.jim.7000163

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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