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Title 

Cloning of the Aspergillus niger pmrA gene, a homologue of yeast PMR1, and characterization of a pmrA null mutant

Authors 

Jae Seung YangKang Hyun AhSu Min KoSuhn Kee ChaeDewey D Y RyuJeong Yoon Kim

Publisher 

Wiley-Blackwell

Issue Date 

2001

Citation 

FEMS Microbiology Letters, vol. 199, no. 1, pp. 97-102

Keywords 

aspergillus nigerpmrA-disrupted mutantCa2+ and Mn2+ homeostasissecretory pathway Ca2+-ATPaseamino acid sequence

Abstract 

The pmrA gene, a yeast PMR1 homologue, was isolated from Aspergillus niger. Sequence analysis of the pmrA cDNA and the genomic DNA revealed that two introns exist in the coding region, and that an open reading frame in the cDNA encodes a polypeptide of 1056 amino acids containing all the conserved regions present in P-type Ca2+-ATPases. The predicted pmrA protein exhibited a high degree of sequence similarity to the Pmr1 proteins from yeasts and mammalians (50-59% identity). The expression of the pmrA cDNA partially restored the growth defect of Yarrowia lipolytica pmr1 null mutant on EGTA-containing medium. This indicates that the A. niger pmrA gene encodes a functional homologue of the yeast P-type Ca2+-ATPase involved in the secretory pathway. An A. niger pmrA null mutant exhibited growth retardation on EGTA-containing medium and the growth defect was overcome by adding Ca2+ or Mn2+ into the medium. This suggests an involvement of the pmrA protein in Ca2+ and Mn2+ homeostasis in A. niger.

ISSN 

0378-1097

Link 

http://dx.doi.org/10.1016/S0378-1097(01)00161-6

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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