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Title 

Isolation and characterization of a Bacillus thuringiensis ssp. kurstaki strain toxic to Spodoptera exigua and Culex pipiens

Authors 

I H LeeY H JeJ H ChangJ Y RohHyun Woo OhS G LeeS C ShinK S Boo

Publisher 

Springer Verlag (Germany)

Issue Date 

2001

Citation 

Current Microbiology, vol. 43, no. 3, pp. 284-287

Keywords 

bacillus thuringiensisbacterium isolationculex pipienslepidopteranonhumanpolyacrylamide gel electrophoresispolymerase chain reactionpriority journaltoxicityanimals

Abstract 

A strain of Bacillus thuringiensis with dual toxicity was isolated from Korean soil samples and named K2. K2 was determined as ssp. kurstaki (H3a3b3c) by serological test and produced bipyramidal-shaped parasporal inclusions. The plasmid and protein profiles of B. thuringiensis K2 were different from those of the reference strain, ssp. kurstaki HD-1. To verify gene type of B. thuringiensis K2, PCR analysis with specific cry gene primers was performed. The result showed that B. thuringiensis K2 had cry1Aa, cry1Ab, cry1C, and cry1D type genes, whereas ssp. kurstaki HD-1 had cry1Aa, cry1Ab, cry1Ac, and cry2 type genes. In addition, B. thuringiensis K2 had high toxicity against Spodoptera exigua and Culex pipiens, whereas B. thuringiensis ssp. kurstaki HD-1 does not have high toxicity against these two insect species.

ISSN 

0343-8651

Link 

http://dx.doi.org/10.1007/s002840010302

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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