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Title 

Typical demethylation events in cloned pig embryos : clues on specis-specific differnces in epigenetic reprogramming of a cloned donor genome

Authors 

Yong Kook KangDeog Bon KooJung Sun ParkYong Hee ChoiHa Na KimWon Kyong ChangKyung Kwang LeeYong Mahn Han

Publisher 

American Society for Biochemistry and Molecular Biology

Issue Date 

2001

Citation 

Journal of Biological Chemistry, vol. 276, no. 43, pp. 39980-39984

Keywords 

demethylationembryopreimplantation embryo

Abstract 

We investigated whether a genomic demethylation process occurs in pig preimplantation embryos produced by either normal fertilization or somatic cell nuclear transfer. The methylation status of the centromeric satellite and the PRE-1 short interspersed element (SINE) sequences was characterized using bisulfite-sequencing technology. Typical demethylation processes were identified in these repetitive sequences of the cloned donor genome during cleavage, the patterns of which were similar to the ones detected in fertilized counterparts. These findings are different from previous observations with cloned bovine embryos where various repeated regions of the donor genome exhibited aberrant methylation patterns. Our results indicate that species-specific differences exist in modifying the epigenetic status of cloned donor genomes.

ISSN 

0021-9258

Link 

http://dx.doi.org/10.1074/jbc.M106516200

Appears in Collections

1. Journal Articles > Journal Articles

Registered Date

2017-04-19


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